Journal: Cancers

Article Title: Carbonic Anhydrase Inhibitors Induce Ferroptosis through Inhibition of AKT/FTH1 Signaling in Ewing Sarcoma Tumor Cells

doi: 10.3390/cancers15215225

Figure Lengend Snippet: Identification of CA-mRNAs in the tissue of ES patients. ( A ) Box plots show the level of CA-mRNA expression in the ES tumor tissue of patients with relapse (red bar) and no relapse (black bar). Two-way ANOVA, Bonferroni test, p = 0.0088 for CA2 mRNA; ( B ) Kaplan–Meier plots for overall survival of the patients with ES using the GSE63155 dataset. Patients were classified according to CA2 mRNA expression (as described in Methods section). Log-rank test p value 0.081; ( C ) Box plots show the level of CAI, CAII, CAVIII, CAX and CAXII protein expression using primary paraffin-embedded ES tumor sections (N = 9) via immunohistochemistry. Kruskal–Wallis test p = 0.0031; PPI network constructed using STRING software (STRING: functional protein association networks ( www.string-db.org )). for the 50 most differentially expressed and top21 ( D ) proteins exhibiting highest degree of correlation with CAII (Spearman degree of correlation between 7.2 × 10 14 till 5.2 × 10 14 ) were used.

Article Snippet: Prior to staining, the working dilution for CAI (Cusabio, Cat # CSB-PA004364ESR1HU, 1/200), CAII (FineTest, Cat #FNab01161, 1/200), CAVIII (Cusabio, Cat # CSB-PA004379ESR2HU, 1/100), CAX (Cusabio, Cat # CSB-PA878879EA01HU, 1/400) and CA12 (Cusabio, Cat # CSB-PA060116, 1/50) antibodies was assessed using tumor sections of human renal cell adenocarcinoma primary tissue embedded in paraffin.

Techniques: Expressing, Immunohistochemistry, Construct, Software, Functional Assay

Journal: bioRxiv

Article Title: Pharmacological inhibition of 11ßhydroxysteroid dehydrogenase type 1 after myocardial infarction targets extracellular matrix processing and preserves cardiac function in a translational mini-pig model

doi: 10.1101/2024.12.02.626322

Figure Lengend Snippet: Representative images from CD163 +ve (a, stars) and fibroblast activation protein (FAP) +ve (b, arrows) cells in the remote undamaged myocardium and infarct of vehicle (n=6) and 11βHSD1i (n=8) 28d post-MI. 11βHSD1i detection via MALDI imaging (c) corresponds with activated fibroblasts in the infarct area (d). Data are presented as mean±SEM. One-tailed unpaired t-test. Scale bar: 50µm (a-b), 2mm (c-d). Heat map (0% black to 100% white) represents intensity of 11βHSD1i signal at 420.2265±0.002 Da.

Article Snippet: The slides were then incubated with rabbit anti-CD31 (Abcam, ab28364, 1:500 in PBS), both anti-CD31 and mouse anti-αSMA (Sigma, A2547, 1:5,000 in PBS), rabbit anti-FAP (Abcam, ab207178, 1:500 in PBS) or mouse anti-CD163 (Novus Biological, NB110-40686, 1:500 in PBS) overnight at 4°C.

Techniques: Activation Assay, Imaging, One-tailed Test